SidaGhanaV1, Main, Exploration, bibRecord, 001481

Biological characterization of human immunodeficiency virus type 1 and type 2 mutants in human peripheral blood mononuclear cells

Identifieur interne : 001481 ( Main/Exploration ); précédent : 001480; suivant : 001482

Biological characterization of human immunodeficiency virus type 1 and type 2 mutants in human peripheral blood mononuclear cells

Auteurs : H. Akari [Japon] ; J. Sakuragi ; Y. Takebe [Japon] ; K. Tomonaga ; M. Kawamura ; M. Fukasawa ; T. Miura ; T. Shinjo [Japon] ; M. Hayami

Source :

Archives of Virology [ 0304-8608 ] ; 1992-03-01.

RBID : ISTEX:4D4EEBB15A120CC49AA38C1FAE57CE835767985A

English descriptors

KwdEn :
- Acad, Akari, Cell lines, Clone, Equivalent amount, Gene product, Growth kinetics, Haseltine, Human immunodeficiency virus, Human immunodeficiency virus type, Immunodeficiency, Insertion, Kinetics, Kyoto university, Molt3 cells, Mutant, Mutation, Mutational analysis, Natl, Nefgene, Negative control, Pbmc, Plasmid, Proc, Proc natl acad, Replication, Syncytium, Syncytium formation, Transfected, Virol, Virus, Virus replication, Wild type, Wild type clone.
Teeft :
- Acad, Akari, Cell lines, Clone, Equivalent amount, Gene product, Growth kinetics, Haseltine, Human immunodeficiency virus, Human immunodeficiency virus type, Immunodeficiency, Insertion, Kinetics, Kyoto university, Molt3 cells, Mutant, Mutation, Mutational analysis, Natl, Nefgene, Negative control, Pbmc, Plasmid, Proc, Proc natl acad, Replication, Syncytium, Syncytium formation, Transfected, Virol, Virus, Virus replication, Wild type, Wild type clone.

Abstract

Summary: Mutants of human immunodeficiency virus type 1 (HIV-1) and type 2 (HIV-2), which have been shown to be infectious in established cell lines, were tested for ability to replicate and induce syncytium formation in human peripheral blood mononuclear cells (PBMC). Thevpu mutant of HIV-1 showed depressed kinetics of replication in an established T cell line, as reported previously, but in PBMC, its replication was similar to that of the wild type virus. Thevpx gene of HIV-2 was required for efficient virus propagation in PBMC, but not in an established T cell line, as previously reported. However, the growth rates of thevpx mutant in PBMC preparations from two individuals were different. The results of experiments on infection of PBMC with thevif andvpr mutants of HIV-1 and HIV-2 were essentially consistent with previous results of infection of established T cell lines. No negative effect of thenef gene products of HIV-1 and HIV-2 was observed. The abilities of the wild type virus and the mutants of HIV-1 to induce syncytium formation in both PBMC and established cell lines were similar. In contrast, neither the wild type nor any of the mutants of HIV-2 induced syncytium formation in PBMC. These results suggest that the functions of some genes can be detected only in mixed populations or primary cells such as PBMC. Studies on the roles of these genes in PBMC may provide a better understanding of their functions in vivo.

Url:

https://api.istex.fr/document/4D4EEBB15A120CC49AA38C1FAE57CE835767985A/fulltext/pdf

DOI: 10.1007/BF01317146

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Istex, to step Corpus: 000083
to stream Istex, to step Curation: 000083
to stream Istex, to step Checkpoint: 000908
to stream Main, to step Merge: 001607
to stream Main, to step Curation: 001481

Le document en format XML

<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Biological characterization of human immunodeficiency virus type 1 and type 2 mutants in human peripheral blood mononuclear cells</title>
<author><name sortKey="Akari, H" sort="Akari, H" uniqKey="Akari H" first="H." last="Akari">H. Akari</name>
</author>
<author><name sortKey="Sakuragi, J" sort="Sakuragi, J" uniqKey="Sakuragi J" first="J." last="Sakuragi">J. Sakuragi</name>
</author>
<author><name sortKey="Takebe, Y" sort="Takebe, Y" uniqKey="Takebe Y" first="Y." last="Takebe">Y. Takebe</name>
</author>
<author><name sortKey="Tomonaga, K" sort="Tomonaga, K" uniqKey="Tomonaga K" first="K." last="Tomonaga">K. Tomonaga</name>
</author>
<author><name sortKey="Kawamura, M" sort="Kawamura, M" uniqKey="Kawamura M" first="M." last="Kawamura">M. Kawamura</name>
</author>
<author><name sortKey="Fukasawa, M" sort="Fukasawa, M" uniqKey="Fukasawa M" first="M." last="Fukasawa">M. Fukasawa</name>
</author>
<author><name sortKey="Miura, T" sort="Miura, T" uniqKey="Miura T" first="T." last="Miura">T. Miura</name>
</author>
<author><name sortKey="Shinjo, T" sort="Shinjo, T" uniqKey="Shinjo T" first="T." last="Shinjo">T. Shinjo</name>
</author>
<author><name sortKey="Hayami, M" sort="Hayami, M" uniqKey="Hayami M" first="M." last="Hayami">M. Hayami</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:4D4EEBB15A120CC49AA38C1FAE57CE835767985A</idno>
<date when="1992" year="1992">1992</date>
<idno type="doi">10.1007/BF01317146</idno>
<idno type="url">https://api.istex.fr/document/4D4EEBB15A120CC49AA38C1FAE57CE835767985A/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000083</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000083</idno>
<idno type="wicri:Area/Istex/Curation">000083</idno>
<idno type="wicri:Area/Istex/Checkpoint">000908</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000908</idno>
<idno type="wicri:doubleKey">0304-8608:1992:Akari H:biological:characterization:of</idno>
<idno type="wicri:Area/Main/Merge">001607</idno>
<idno type="wicri:Area/Main/Curation">001481</idno>
<idno type="wicri:Area/Main/Exploration">001481</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Biological characterization of human immunodeficiency virus type 1 and type 2 mutants in human peripheral blood mononuclear cells</title>
<author><name sortKey="Akari, H" sort="Akari, H" uniqKey="Akari H" first="H." last="Akari">H. Akari</name>
<affiliation></affiliation>
<affiliation wicri:level="1"><country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Veterinary Medicine, Faculty of Agriculture, Miyazaki University, Miyazaki</wicri:regionArea>
<wicri:noRegion>Miyazaki</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Biodefence and Medical Virology, Kumamoto University Medical School, 860, Kumamoto</wicri:regionArea>
<wicri:noRegion>Kumamoto</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Sakuragi, J" sort="Sakuragi, J" uniqKey="Sakuragi J" first="J." last="Sakuragi">J. Sakuragi</name>
<affiliation><wicri:noCountry code="subField">Kyoto</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Takebe, Y" sort="Takebe, Y" uniqKey="Takebe Y" first="Y." last="Takebe">Y. Takebe</name>
<affiliation wicri:level="3"><country>Japon</country>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
<wicri:orgArea>AIDS Research Center, National Institute of Health</wicri:orgArea>
</affiliation>
</author>
<author><name sortKey="Tomonaga, K" sort="Tomonaga, K" uniqKey="Tomonaga K" first="K." last="Tomonaga">K. Tomonaga</name>
<affiliation><wicri:noCountry code="subField">Kyoto</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Kawamura, M" sort="Kawamura, M" uniqKey="Kawamura M" first="M." last="Kawamura">M. Kawamura</name>
<affiliation><wicri:noCountry code="subField">Kyoto</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Fukasawa, M" sort="Fukasawa, M" uniqKey="Fukasawa M" first="M." last="Fukasawa">M. Fukasawa</name>
<affiliation><wicri:noCountry code="subField">Kyoto</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Miura, T" sort="Miura, T" uniqKey="Miura T" first="T." last="Miura">T. Miura</name>
<affiliation><wicri:noCountry code="subField">Kyoto</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Shinjo, T" sort="Shinjo, T" uniqKey="Shinjo T" first="T." last="Shinjo">T. Shinjo</name>
<affiliation wicri:level="1"><country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Veterinary Medicine, Faculty of Agriculture, Miyazaki University, Miyazaki</wicri:regionArea>
<wicri:noRegion>Miyazaki</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hayami, M" sort="Hayami, M" uniqKey="Hayami M" first="M." last="Hayami">M. Hayami</name>
<affiliation><wicri:noCountry code="subField">Kyoto</wicri:noCountry>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Archives of Virology</title>
<title level="j" type="abbrev">Archives of Virology</title>
<idno type="ISSN">0304-8608</idno>
<idno type="eISSN">1432-8798</idno>
<imprint><publisher>Springer-Verlag</publisher>
<pubPlace>Vienna</pubPlace>
<date type="published" when="1992-03-01">1992-03-01</date>
<biblScope unit="volume">123</biblScope>
<biblScope unit="issue">1-2</biblScope>
<biblScope unit="page" from="157">157</biblScope>
<biblScope unit="page" to="167">167</biblScope>
</imprint>
<idno type="ISSN">0304-8608</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0304-8608</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acad</term>
<term>Akari</term>
<term>Cell lines</term>
<term>Clone</term>
<term>Equivalent amount</term>
<term>Gene product</term>
<term>Growth kinetics</term>
<term>Haseltine</term>
<term>Human immunodeficiency virus</term>
<term>Human immunodeficiency virus type</term>
<term>Immunodeficiency</term>
<term>Insertion</term>
<term>Kinetics</term>
<term>Kyoto university</term>
<term>Molt3 cells</term>
<term>Mutant</term>
<term>Mutation</term>
<term>Mutational analysis</term>
<term>Natl</term>
<term>Nefgene</term>
<term>Negative control</term>
<term>Pbmc</term>
<term>Plasmid</term>
<term>Proc</term>
<term>Proc natl acad</term>
<term>Replication</term>
<term>Syncytium</term>
<term>Syncytium formation</term>
<term>Transfected</term>
<term>Virol</term>
<term>Virus</term>
<term>Virus replication</term>
<term>Wild type</term>
<term>Wild type clone</term>
</keywords>
<keywords scheme="Teeft" xml:lang="en"><term>Acad</term>
<term>Akari</term>
<term>Cell lines</term>
<term>Clone</term>
<term>Equivalent amount</term>
<term>Gene product</term>
<term>Growth kinetics</term>
<term>Haseltine</term>
<term>Human immunodeficiency virus</term>
<term>Human immunodeficiency virus type</term>
<term>Immunodeficiency</term>
<term>Insertion</term>
<term>Kinetics</term>
<term>Kyoto university</term>
<term>Molt3 cells</term>
<term>Mutant</term>
<term>Mutation</term>
<term>Mutational analysis</term>
<term>Natl</term>
<term>Nefgene</term>
<term>Negative control</term>
<term>Pbmc</term>
<term>Plasmid</term>
<term>Proc</term>
<term>Proc natl acad</term>
<term>Replication</term>
<term>Syncytium</term>
<term>Syncytium formation</term>
<term>Transfected</term>
<term>Virol</term>
<term>Virus</term>
<term>Virus replication</term>
<term>Wild type</term>
<term>Wild type clone</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Summary: Mutants of human immunodeficiency virus type 1 (HIV-1) and type 2 (HIV-2), which have been shown to be infectious in established cell lines, were tested for ability to replicate and induce syncytium formation in human peripheral blood mononuclear cells (PBMC). Thevpu mutant of HIV-1 showed depressed kinetics of replication in an established T cell line, as reported previously, but in PBMC, its replication was similar to that of the wild type virus. Thevpx gene of HIV-2 was required for efficient virus propagation in PBMC, but not in an established T cell line, as previously reported. However, the growth rates of thevpx mutant in PBMC preparations from two individuals were different. The results of experiments on infection of PBMC with thevif andvpr mutants of HIV-1 and HIV-2 were essentially consistent with previous results of infection of established T cell lines. No negative effect of thenef gene products of HIV-1 and HIV-2 was observed. The abilities of the wild type virus and the mutants of HIV-1 to induce syncytium formation in both PBMC and established cell lines were similar. In contrast, neither the wild type nor any of the mutants of HIV-2 induced syncytium formation in PBMC. These results suggest that the functions of some genes can be detected only in mixed populations or primary cells such as PBMC. Studies on the roles of these genes in PBMC may provide a better understanding of their functions in vivo.</div>
</front>
</TEI>
<affiliations><list><country><li>Japon</li>
</country>
<region><li>Région de Kantō</li>
</region>
<settlement><li>Tokyo</li>
</settlement>
</list>
<tree><noCountry><name sortKey="Fukasawa, M" sort="Fukasawa, M" uniqKey="Fukasawa M" first="M." last="Fukasawa">M. Fukasawa</name>
<name sortKey="Hayami, M" sort="Hayami, M" uniqKey="Hayami M" first="M." last="Hayami">M. Hayami</name>
<name sortKey="Kawamura, M" sort="Kawamura, M" uniqKey="Kawamura M" first="M." last="Kawamura">M. Kawamura</name>
<name sortKey="Miura, T" sort="Miura, T" uniqKey="Miura T" first="T." last="Miura">T. Miura</name>
<name sortKey="Sakuragi, J" sort="Sakuragi, J" uniqKey="Sakuragi J" first="J." last="Sakuragi">J. Sakuragi</name>
<name sortKey="Tomonaga, K" sort="Tomonaga, K" uniqKey="Tomonaga K" first="K." last="Tomonaga">K. Tomonaga</name>
</noCountry>
<country name="Japon"><noRegion><name sortKey="Akari, H" sort="Akari, H" uniqKey="Akari H" first="H." last="Akari">H. Akari</name>
</noRegion>
<name sortKey="Akari, H" sort="Akari, H" uniqKey="Akari H" first="H." last="Akari">H. Akari</name>
<name sortKey="Shinjo, T" sort="Shinjo, T" uniqKey="Shinjo T" first="T." last="Shinjo">T. Shinjo</name>
<name sortKey="Takebe, Y" sort="Takebe, Y" uniqKey="Takebe Y" first="Y." last="Takebe">Y. Takebe</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/SidaGhanaV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001481 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001481 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Sante
   |area=    SidaGhanaV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     ISTEX:4D4EEBB15A120CC49AA38C1FAE57CE835767985A
   |texte=   Biological characterization of human immunodeficiency virus type 1 and type 2 mutants in human peripheral blood mononuclear cells
}}

This area was generated with Dilib version V0.6.31.
Data generation: Tue Nov 7 18:07:38 2017. Site generation: Tue Mar 5 15:01:57 2024

	Le SIDA au Ghana (serveur d'exploration)
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Le SIDA au Ghana (serveur d'exploration)

Biological characterization of human immunodeficiency virus type 1 and type 2 mutants in human peripheral blood mononuclear cells

Biological characterization of human immunodeficiency virus type 1 and type 2 mutants in human peripheral blood mononuclear cells

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri